Aria exus user manual rev b 1140201 4 using your aria your aria scale measures your weight and body fat percentage. The procedures outlined in this sop are a supplement to general laboratory safety procedures outlined in the uw biosafety manual. The instrument is able to perform two and fourway aseptic sorting for a variety of tube sizes as well as sorting into multiwell plates. Facsaria fusion sorp quick reference ut southwestern. For a full description of the cytometer status report, refer to the bd facsdiva software reference manual. Becton dickinson pty ltd, 4 research park drive, macquarie university research park, north ryde nsw 21, australia becton dickinson limited, 8 pacific rise, mt. The bd facsaria ii was set for highspeed sorting 70 psi and 90 khz and both instruments were set up using bd cytometer setup and tracking software. Bd facsversetm operator course course objectives practical sessions cover.
Childrens healthcare of atlanta, emory university school of medicine, and georgia institute of technology have formed a close partnership that combines the clinical strengths of the nations largest pediatric healthcare provider childrens, the research strengths of a leading. The bd facsaria iii sorter is built on the solid foundation of patented technologies, superior multicolor performance, and legendary easeofuse that opened the complex world of cell sorting to a broader audience of researchers and wider range of applications. Stream breakoff and droplet formation 100m nozzle a. Our technological advances in flow cytometry help customers advance their research and discovery. Ensure that the center stream is bright, tight, and. Feb 02, 2021 we are bostonlocated flow cytometry core facility equipped with analyzers 5laser bd lsr ii sorp, a facscanto and a facscalibur and cell sorters 4 laser16 color facs aria ii sorp and a 5 laser25 color moflo astrios. Facsaria i standard operation protocol basic operation. You will see the most consistent and reliable readings when placing your aria scale on a hard, flat surface. Dec 09, 2019 preparation, transport, and use of samples using a becton dickinson bd biosciences facs aria iii cell sorter with aerosol management system ams for work in the flow cytometry facility. Instrument standard filter set all flow core cytometers including the imagestream. Safety and limitations the bd facsaria flow cytometer is equipped with safety features for your protection. Log in to the computer facsuser, password bdis and open bd facsdiva software. The bd facsaria iii sorter is built on the solid foundation of patented technologies, superior multicolor performance, and legendary easeofuse that opened.
Based on an entirely new design in instrumentation, the bd facsaria instrument is the first benchtop sorter that incorporates a fixedalignment cuvette flow cell. The bd facsaria cell sorter embodies this name because it is. Bd facs aria startup guide and sop startup guide and sop a 12,000volt potential exists between the deflection plates when they are on. Volumes are drastically reduced, typically a 5 ml facs tube will hold 5 million cells. Bd biosciences does not make any warranty with respect to the workstation remaining virus free after installation. Do not touch the deflection plates when the voltage warning light is illuminated, or when the software indicates that the plate voltage is. Your lab will just be charged for the time spent on the aria at our normal sort rate for all phases of this program. Feb 17, 2017 childrens research institute at utsw flow cytometry shared facility last update. Bd facs aria startup guide and sop stem cell instrumentation.
Innovative designs for both the excitation optics and collection optics reduce excitation losses and dramatically improve collection efficiency. This users guide contains the instructions necessary to operate and maintain your bd facsaria flow cytometer. Remove the cst beads from the loading port, exit the cst software, and allow the instrument to reconnect to diva. Log in to diva at the prompt with your diva username and password. Sort operators should be trained in use and maintenance of the instruments, as well as scfc. Replace the close loop nozzle with the appropriate one if analysis use 70m 3 sizes are currently available. Facsaria sorp standard operation protocol basic operation. University of massachusetts medical school flow cytometry core facility bd facsaria ii selfsorting training program prepared by marc barnard table of contents page 2 introduction page 3 training overview page 4 starting up the system page 5 cleaning and shutting down the system page 6 optimizing the side streams page 7 aiming the streams and facsdiva toolbar guide page 8 setting. Automatic biexponential scaling values can be manually adjusted in the biexponential editor.
Stopping time selection maintains the last entered value. The bd facsaria fusion users guide assumes you have a working knowledge of basic microsoft windows operation. Bd facsaria ii users guide iub flow cytometry core facility. The information in this guide is subject to change without notice. The procedures outlined in this sop are a supplement to general laboratory. A mask is a region of the stream which is monitored for the presence of cells of interest defined by bd biosciences facsaria training manual. Facsaria ii the bd facsaria is a color 15parameter custommade highspeed digital benchtop cell sorter equipped with four adjustable excitation lasers 100 mw blue, 50 mw 561nm green, 100 mw red and 100 mw violet. Biexponential display is enabled by default, but can be disabled in user preferences, allowing more events to be recorded per experiment.
The bd facsaria cell sorter is named for the musical term. Instruments and services flow cytometry core facility. The results especially for single cell sorting are amazing. A highthroughput automated platform for the development of. As with all bd instruments, the bd facslyric is backed by 40 years of bd expert training, service and support so theres no limit to your potential. Faculty core facility, li ka shing faculty of medicine.
Bd facsaria sorp operation manual 2 power of blue laser is not optional. The bd facsaria fusion flow cytometer delivers exceptional multicolor performance and easeofuse. Bd facsaria manuals manuals and user guides for bd facsaria. With the aria ii, it should not be necessary to wiggle the nozzle. After waiting for a period of time for the stream to stabilize, enter by hand the.
Bd facsaria quick reference guide use this quick reference guide once you are familiar with the procedures in the bd facsaria users guide. Core cytek aurora university of massachusetts medical school. Fragile cells do better with a larger nozzle but sorting speed would be faster with the 70m nozzle. Bd facs accudrop beads are used to automatically set an accurate drop delay value. Bd biosciences is not liable for any claims related to or resulting from buyerusers failure to install and maintain virus protection. The bd facsaria tm fusion has been verified to meet personnel and product protection standards for a class ii type a2 biosafety cabinet, the national sanitation foundation international standard 49, the european standard 12469, and the australian standard as 2252. Uv laser excites at 355nm, it may cause dna damage during sorting experiment. The bd facs melody is a small and efficient cell sorter intended for relatively simple sorting applications our melody is configured with blue 488 nm, yellowgreen 561 nm, and violet 405 nm lasers plus photomultiplier tubes for the detection of up to 8 fluorescent parameters, including common fluorophores like egfp and mcherry along with dead cell discrimination markers like dapi or live. We recommend that participants have completed the introduction to flow cytometry, a selfpaced elearning course that takes you through the general concepts and principles behind flow cytometry. Postsort instrument cleaning and shutdown will be performed. We have 1 bd facsaria manual available for free pdf download. University of massachusetts medical school flow cytometry. We have facsdiva, cellquest pro and flowjo analysis stations. In this operation, production host cells are first transfected with an expression vector carrying the gene of interest 1, followed by the treatment with a selection agent.
Bd facsdiva software contains vxworks as embedded software runtime module. Networking requirements the fitbit aria connects directly to your homes wireless network and supports the following network configurations. The accudrop laser is aligned with the center and side sorting streams. When connected to your account, weight and body fat measurements from your scale will automatically sync to your dashboard. For more details, refer to chapter 4 in the users guide. Facsaria i standard operation protocol basic operation ispybio. Contact with the charged plates results in serious electrical shock. Lab c5027, winship building c 4047276852 cytek aurora. Pediatric research is thriving and expanding in atlanta through a unique, multiinstitutional initiative.
The following steps are prepared for cell sorting using 100m nozzle. Built on a foundation of excellence, experience and expertise, the bd facslyric is a new diagnostic standard for clinical cell analysis, transforming the way your lab does flow cytometry. The combination of bd facs aria cell sorter, cloneselect imager and tecan freedom evo liquid handling system has enabled a highthroughput and more efficient cell line development process. Introduction to flow aria fortessacantoaccuri laserpmt comp beads insert instructions selecting reagents for multicolor flow cytometry bd technotes titration of antibodies protocol titration of antibodies. Facs aria sorp bd page content the faculty of medicine is proud to announce that the facs core facility includes a state of the art facs sorter. The bd facsaria may be autonomously run by appropriately trained users. Lab policies and procedures, instrument setupmonitoring community texts 8 8. Atlanta pediatric research instruments flow cytometry. University of massachusetts medical school flow cytometry core facility bd facsaria ii selfsorting training program prepared by marc barnard table of contents page 2 introduction page 3 training overview page 4 starting up the system page 5 cleaning and shutting down the system page 6 optimizing the side streams page 7 aiming the streams and facsdiva toolbar guide page 8 setting drop delay.
User guides and information flow cytometry core facility. The numerous technological advances embodied in the bd facsaria cell sorter reduce the cost of owning a highspeed sorter and accelerate. The new bd facsaria ii cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. Viewing your data when you have weighed yourself and your aria has synced, you can view the. Bd facsaria quick reference guide biosciences area. In this operation, production host cells are first transfected with an expression vector carrying the gene of interest 1, followed by the treatment with a. Bd falcon 50ml tubes with fliptop cap for easy onehanded opening and closing also in this issue, find an interview with timothy bushnell, director of the cpbr flow cytometry laboratory at the university of rochester, new york speaking about his work and the impact flow cytometry has had on scientific discovery. Adjust the frequency freq between 27 and 30 to identify the highest breakoff, where the endportion bottom neck of the breaking stream can still be observed. For detailed information on software features, refer to the bd facsdiva software reference manual. Events to record field maintains the last entered value.
Wellington, auckland, new zealand ec rep bd facsdiva software reference manual for version 7. Turn on the stream and allow time for it to stabilize. If you are not familiar with the windows operating system, refer to the documentation provided with your computer. Dashboard on page 128 in the bd facsdiva software reference manual. Bd facsaria cellsorting system from bd biosciences. Yield is slightly decreased typically you loose 1% events compared to purity. Running a basic 2 color flow cytometry experiment in bd. Ensure that the sorter has been prepared for the day by cleaning and running quality control. Running a basic 2 color flow cytometry experiment in bd facs. Information about how to start up and shutdown 4 laser bd facs aria by user topics. First introduced as the bd facsaria sorter in 2003, this sorting knowhow is combined with bestinclass biosafety expertise for a comprehensive advanced cell sorter and biosafety solution. Childrens research institute at utsw flow cytometry 2shared facility last update. Masks divide cells into 32 equal segments, and choosing different types of available masks determines how drops will be deflected if a sorting conflict occurs bd biosciences facsaria training manual.
A bd facsaria cytometer report includes the following additional information. Pages 16 aria iii usz sep 2020 general note only power users having passed the sorter training at the cytometry facility are allowed to operate the aria iii cell sorter. Sep 22, 2011 the combination of bd facs aria cell sorter, cloneselect imager and tecan freedom evo liquid handling system has enabled a highthroughput and more efficient cell line development process. If possible, reduce the center core stream bead spot brightness to minimize the discrepancy amongst the center core stream and the other four. If the sweet spot is off, breakoff and gap values are shown. See acquisition setup on page 1 in the bd facsdiva software reference manual. For detailed information on software functions, see the bd facsdiva software reference manual. For detailed information on software features, refer to the bd facsdiva. The bd facsaria cell sorter is named for the musical term aria, meaning a striking solo performance.
This video describes how to set up an experiment in facs diva version 8. For information on instrument maintenance, please see the individual instrument sop. Adjust the micrometer dial next to the sort block, if necessary, to obtain evenly brightest bead spots for the four side streams. Bd facs melody temerty faculty of medicine flow cytometry. Flow cytometry core facility bd facsaria ii selfsorting training program prepared by marc barnard. Its not too easy to use, not for beginners, but if you already work on a cell sorter, this is the best. The new facs aria iii is the most powerful cytofluorimeter that i ever tested. The heart of this cell sorting revolution is the true fixedalignment flow cell, gelcoupled to the collection optics. Adjust the frequency freq between 27 and 30 to identify the highest breakoff, where the endportion of the breaking stream can still be observed. Optics excitation optics optical platform fixed optical alignment of all class iiib lasers upon the cuvette flow cell.
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